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Copyright 2012: Nature’s Fluid. Organic Plant Fertilizer. All Rights Reserved.
7645 CR 3600 Bownsboro, TX. 77327 850-830-1624

Green House Study


Green House Study of Product (Nature’s Fluid )
 A. Williams PhD 
Crop : Common field corn seed

A Green House Study was performed using a Common Field Corn Seeds using a Proprietary Micro Nutrient Root Stimulator for plant growth.
By A. Williams PhD
Introduction 
Seed germination observation has been used for thousands of years. Since mankind has been on earth. The seed study have been going on for hundreds of years and science have it where you can predict the outcome of plant for crop production based on how will the seeds preformed in a study. Normally studies of seed production are done in labs or in green houses in a control condition to maximize and optimize the condition to get better production can be used in the field environment for crop production. 
Location of Study: Southern University A&M College in Baton Rouge, La.
The study was setup on September 8, 2010 at there on campus green house facility. This study was performed by students in the undergraduates and graduate programs in Urban forest and agriculture sciences at Southern University A&M Baton Rouge, La to give the students hand on experiences of entrepreneurship through sciences. This study was design by Williams Strange and Aubrey Williams PhD. 
Objective of Study
Objective of Study were to use this proprietary micro nutrient root stimulator to grow corn from seeds to plants and to verify the label parameter for use of the product.
The label parameters are:  
1.This product is highly effective to use when plants are struggling and a fast boost to get through stress conditions is desired. Nature’s Fluid will enter the plant within 3-4 hours quickly and effectively mobilizing nutrients to show response within 24 hours.
2.The micro nutrients in Nature’s Fluid will feed the plant for approximately 7 day making this an excellent natural plant stimulant addition to a balanced foliar fertility program for many flowers, fruits, and vegetables. 
3.Nature’s Fluid promotes new growth, a blooming agent, a rooting agent and soil stimulate. 

Experiment data parameters:
 The experiment parameters were plant height, chlorophyll reading, and root mass observation and application frequency. 


Procedure of the study
The Experiment Design was a random complete block 4 x4. There will be four treatments and four reps. The treatments were a comparison of the proprietary micro nutrient root stimulator Nature fluid (NF) verse Tap water. The seed was a common field corn seed. 
 Procedure of the study preparation on setup:

1) Pretreatment of the seeds was not done on this study but is recommended and in the future 
  will be done.  
2) Soil garden mix soil  
3) Plant seed in a one gallon pot and apply treatment (NF) to the soil over the seeds
4) Every week apply the treatment (NF) to each pot when watering them from the seed mature  
  to plants.

Watering of plants and proprietary micro nutrient root stimulator application
A total of 400 ml of water treatment was added to the plants when watered. For treatment and water the amount of water and treatment will be added to give the plant the multiple application of the stimulant that is need to show it effectiveness. Continue to water like this until the mature of the plant until harvesting. Replications 1, 2, 3, 4 and Treatments 1,2,3,4 (below)
Treatment 1 56 ml of NF add water to 400 ml in the beaker 
Treatment 2 113 ml of NF add water to 400 ml in the beaker 
Treatment 3 225 ml of NF add water to 400 ml in the beaker
Treatment 4 water (control) is add the same amount add water as the other rep’s.  

Data that will be gathered from study will be visual growth rate in inches, chlorophyll readings (spad) and root density by treatment.  








Discussion 
Objective of Study were to use this proprietary micro nutrient root stimulator to grow corn from seeds to plants and to verify the label parameter for use of the product. 
Label parameter: One
“This product is highly effective to use when plants are struggling and a fast boost to get through stress conditions is desired. Nature’s Fluid will enter the plant within 3-4 hours quickly and effectively mobilizing nutrients to show response within 24 hours.”
The corn plants were stress by not watering or applying treatment (NF) to the plants for twelve days to the plants from September 20 thru October 3, 2010. This was not a planned event but, it worked out in the favor of the product used. 
On October 3, 2010 we thinned the plants out from five corn plants to two corn plants; the product was applied at the rates for the treatments 1, 2, 3, and 4. During this time we also, took the plants height and chlorophyll measurements.
The plant height for October 3 was for treatment 1, 2, 3, 4, and 14.0, 14.15, 14.30, 14.12 inches respectively. The next day, October 4 in twenty four hours later , treatment 1, 2, 3, 4, and 14.25, 14.62, 14.62, 14.5 inches respectively. All treatments had improved in height in twenty four hours about eighth of an inch or more. (Table one)
Table One Plant height measurement for all treatments had improved in height in twenty four hours about eighth of an inch or more.
 PLANT HEIGHT MEASUREMENT  
Date of collection15-Sep20-Sep3-Oct4-Oct8-Oct20-Oct
Days of Growth81326273143
Treatments Rate NFinches  
T156 ml4.010.314.014.315.316.0
T2113 ml4.511.014.214.616.317.3
T3225ml 4.09.614.314.615.616.6
T40 ml4.09.514.114.514.816.5
 All data was from average of four reps 








The chlorophyll readings were taken on October 3 and October 28 for all treatments and replication. For October 3 treatment 1, 2, 3, and 4 were 10.8, 7.8, 8.1, and 8.4 respectively. For October 28 for treatments 1, 2, 3, and 4 were 10.8, 11.0, 8.5, and 10.6 respectively. The chlorophyll reading all had improved after the stress period. (Table two)
Table Two the chlorophyll reading all had improved after the stress period.
Chlorophyll Reading  
Date of collection 20-Sep3-Oct4-Oct28-Oct 
Days of Growth13262751 
Treatments Rate NFSPAD  
T156 ml19.910.88.310.8 
T2113 ml20.97.88.811.0 
T3225ml 19.78.16.88.5 
T40 ml21.58.46.510.6 
 All data was from average of four reps 


Label parameter: Two
“ The micro nutrients in Nature’s Fluid will feed the plant for approximately 7 day making this an excellent natural plant stimulant addition to a balanced foliar fertility program for many flowers, fruits, and vegetables. “
The stress period September 20 thru October 3, 2010 validate that when the product was applied 
to the plant either by soil or by foliage the will show signs of recovery. The plant were health after the treatment and watered was applied there was new growth on all the plants. (Figure 1, 2)
Figure 1, was taken on October 3, 2010 and it was during the stress period. You can see the yellowing of the leaves of the plant and can tell it was dry. 
Figure 2, was taken on October 4 2010 about twenty four hours later and after NF was applied and it greener and healthy looking and hydrated. 


  Figure 1 October 3, 2010

  Figure 2 October 4, 2010

Label parameter: Three
“Nature’s Fluid promotes new growth, a blooming agent, a rooting agent and soil stimulate.” 
The root mass of the corn plants were observed on October 3, 2010 when we thinned out, the plants from five to two plants. We able to observed the plants roots. The reason behind thinning is the plants would have less time competing for the nutrient in soil for plant growth. If too many plants in the pot the nutrient will be uptake unevenly and the true observation could not be determine. From this study the root mass shows the difference. The root mass difference in inches for day 26 of growth for treatments 1, 2, 3, 4 and 56, 113, 225, ml of the product (NF) respectively was for treatment 1,2,3,4, was 3,3,5,2.5 inches respectively. Treatment had the largest root mass of all treatments at 5 inches. Treatment 4 was the control and only water was used. In the first day of growth the plant roots were observed out the pot. (Figure 3,4, 5,6,7)




Figure 3 the first week of growth the roots were coming out of the pot bottom.

Figure 4 treatment 1 56 ml of NF


  Figure 5 treatment 2 113ml of NF

Figure 6 225ml of NF 


Figure 7 water (control)



Conclusion:
The green house study was a success in ways that could not be done in the field. We were able to control and monitor the growth process. We were able to verify the label instruction on the container. Which was not planned in the setup of the study, but it was a success in many ways it gave the students chance to see how science can be used in the real world. The lesson learns is, if nature’s fluid is applied in the frequency that is stated on the label, it will perform according to the label and it clams.  

Recommendation:
In future studies we will use a different soil mix. In this study the treatment effects could not be observed, but in other studies that was done this year it was observed. 
Acknowledgements: 
Thanks to Dr. Yemane Ghebreiyessus Urban Forest Program for allowing us to use the green house at Southern University A & M Baton Rouge, La. 70813